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Journal article

Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR.

Abstract:

A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted NDV-infected allantoic fluid and found to be 10(-5) HA units. Application of one-step RT-PCR to vario...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Onderstepoort journal of veterinary research More from this journal
Volume:
68
Issue:
2
Pages:
131-134
Publication date:
2001-06-01
EISSN:
2219-0635
ISSN:
0030-2465
Language:
English
Keywords:
Pubs id:
pubs:55717
UUID:
uuid:a757c9d2-ef39-4702-9ba7-c35524212b3d
Local pid:
pubs:55717
Source identifiers:
55717
Deposit date:
2012-12-19

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