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Subunit architecture of multimeric complexes isolated directly from cells.

Abstract:

Recent developments in purification strategies, together with mass spectrometry (MS)-based proteomics, have identified numerous in vivo protein complexes and suggest the existence of many others. Standard proteomics techniques are, however, unable to describe the overall stoichiometry, subunit interactions and organization of these assemblies, because many are heterogeneous, are present at relatively low cellular abundance and are frequently difficult to isolate. We combine two existing metho...

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Publication status:
Published

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Publisher copy:
10.1038/sj.embor.7400702

Authors


Hernández, H More by this author
Dziembowski, A More by this author
Taverner, T More by this author
Séraphin, B More by this author
Robinson, CV More by this author
Journal:
EMBO reports
Volume:
7
Issue:
6
Pages:
605-610
Publication date:
2006-06-05
DOI:
EISSN:
1469-3178
ISSN:
1469-221X
URN:
uuid:a697365e-daa7-4f63-b50d-3581ed6efbb5
Source identifiers:
59289
Local pid:
pubs:59289

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