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Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement.

Abstract:

We have examined the role of the human CD59 antigen in inhibiting complement-mediated lysis by transfer and expression of a CD59 cDNA in rat cells. A cDNA encoding CD59 was subcloned into the expression vector pSFSVneo and stably transfected into the rat T cell line NB2-6TG. Indirect immunofluorescence staining using the anti-CD59 monoclonal antibody YTH53.1 demonstrated the presence of human CD59 antigen on transfected cells and its attachment to the cell surface by a rat glycolipid anchor. ...

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Publication status:
Published

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Publisher copy:
10.1002/eji.1830210349

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
European journal of immunology
Volume:
21
Issue:
3
Pages:
847-850
Publication date:
1991-03-05
DOI:
EISSN:
1521-4141
ISSN:
0014-2980
URN:
uuid:a5dd03c9-67da-4988-8826-13c1986c4197
Source identifiers:
19320
Local pid:
pubs:19320

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