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Monitoring triplet state dynamics with fluorescence correlation spectroscopy: Bias and correction

Abstract:

A marker's dark triplet state is of great importance in fluorescence microscopy: It serves as a means to switch off fluorescent markers and is thus the enabling element for several super-resolution methods. On the other hand, intersystem-crossing to the electronic dark triplet state strongly reduces the fluorescence yield in conventional fluorescence microscopy. The ability to determine the kinetic parameters of transitions into the triplet state is thus of great importance and because fluore...

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Publisher copy:
10.1002/jemt.22368

Authors


Publisher:
Wiley-Liss Inc.
Journal:
Microscopy Research and Technique
Volume:
77
Issue:
7
Pages:
528-536
Publication date:
2014-01-01
DOI:
EISSN:
1097-0029
ISSN:
1059-910X
URN:
uuid:a5b0a416-2718-43fd-8cf3-338ba7521a4c
Source identifiers:
474841
Local pid:
pubs:474841

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