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Mapping the 5-50-kDa fraction of human amniotic fluid proteins by 2-DE and ESI-MS.

Abstract:

This report presents a proteomic analysis and provides a reference map of the 5-50-kDa components of normal amniotic fluid collected in gestational weeks 16-18. Early amniocentesis samples were pooled and proteins with molecular mass lower than albumin were separated by gel filtration chromatography. The 2-DE protocol was optimized for the separation of the small proteins and peptides in the fraction of interest. A total of 132 Coomassie blue-stained protein spots were analyzed, following in-...

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Publication status:
Published

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Publisher copy:
10.1002/prca.200600543

Authors


Gianazza, E More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Eberini, I More by this author
Campagnoli, M More by this author
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Journal:
Proteomics. Clinical applications
Volume:
1
Issue:
2
Pages:
167-175
Publication date:
2007-02-05
DOI:
EISSN:
1862-8354
ISSN:
1862-8346
URN:
uuid:a5ab23d2-2c77-4f16-93c1-e17ea02285dc
Source identifiers:
230726
Local pid:
pubs:230726

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