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A yeast artificial chromosome contig containing the complete Duchenne muscular dystrophy gene.

Abstract:

A contig of 36 overlapping yeast artificial chromosome (YAC) clones has been constructed for the complete Duchenne muscular dystrophy (DMD) gene in Xp21. The YACs were isolated from a human 48,XXXX YAC library using the DMD cDNA and brain promoter fragments as hybridization probes. The YAC clones were characterized for exon content using HindIII or EcoRI digests, hybridization of individual DMD cDNA probes, and polymerase chain reaction (PCR) amplification of specific exons near the 5' end of...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Journal:
Genomics
Volume:
12
Issue:
3
Pages:
465-473
Publication date:
1992-03-01
DOI:
EISSN:
1089-8646
ISSN:
0888-7543
Source identifiers:
32932
Language:
English
Keywords:
Pubs id:
pubs:32932
UUID:
uuid:a4b308ab-4ed3-47e3-9d64-031ee38b778e
Local pid:
pubs:32932
Deposit date:
2012-12-19

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