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A physical assay for sister chromatid cohesion in vitro.

Abstract:

Cohesion between sister chromatids depends on a multiprotein complex called cohesin that has been proposed to hold sister DNAs together by trapping them inside a large tripartite ring. Sister chromatid cohesion has hitherto only been detected by using cytological methods in living cells. We show here that cohesion between the sister DNAs of circular minichromosomes established in vivo can be detected in vitro by velocity gradient sedimentation and agarose-gel electrophoresis. This ex vivo coh...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Journal:
Molecular cell
Volume:
27
Issue:
2
Pages:
300-310
Publication date:
2007-07-05
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
URN:
uuid:a458bcee-376a-46fb-97c9-ba771c2b5a47
Source identifiers:
98456
Local pid:
pubs:98456

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