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Multiscale spatial organization of RNA polymerase in Escherichia coli.

Abstract:

Nucleic acid synthesis is spatially organized in many organisms. In bacteria, however, the spatial distribution of transcription remains obscure, owing largely to the diffraction limit of conventional light microscopy (200-300 nm). Here, we use photoactivated localization microscopy to localize individual molecules of RNA polymerase (RNAP) in Escherichia coli with a spatial resolution of ∼40 nm. In cells growing rapidly in nutrient-rich media, we find that RNAP is organized in 2-8 bands. The ...

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Publication status:
Published

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Publisher copy:
10.1016/j.bpj.2013.05.048

Authors


Endesfelder, U More by this author
Holden, SJ More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Kapanidis, AN More by this author
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Journal:
Biophysical journal
Volume:
105
Issue:
1
Pages:
172-181
Publication date:
2013-07-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:a448c232-5691-42e4-87dd-5b8662d381f7
Source identifiers:
410861
Local pid:
pubs:410861

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