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Journal article

Determination of adeno-associated virus rep DNA binding using fluorescence anisotropy.

Abstract:
Quantitative measurement of proteins binding to DNA is a requisite to fully characterize the structural determinants of complex formation necessary to understand the DNA transactions that regulate cellular processes. Here we describe a detailed protocol to measure binding affinity of the adeno-associated virus (AAV) Rep68 protein for the integration site AAVS1 using fluorescent anisotropy. This protocol can be used to measure the binding constants of any DNA binding protein provided the substrate DNA is fluorescently labeled.
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.21769/BioProtoc.2194

Authors


Zarate-Perez, F More by this author
Santosh, V More by this author
More by this author
Department:
Oxford, MSD, NDM, Jenner Institute
Agundez, L More by this author
Linden, RM More by this author
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Publisher:
Bio-Protocol Publisher's website
Journal:
Bio-Protocol Journal website
Volume:
7
Issue:
6
Publication date:
2017-03-05
DOI:
ISSN:
2331-8325
Pubs id:
pubs:702133
URN:
uri:a431a60e-9289-4467-80cb-d0fbfcbd6122
UUID:
uuid:a431a60e-9289-4467-80cb-d0fbfcbd6122
Local pid:
pubs:702133

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