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Photon counting histogram for one-photon excitation.

Abstract:

An advance that counts! A method is presented for analyzing single-molecule behavior using one-photon excitation of fluorescence. It is based on the photon counting histogram (PCH) procedure that has enjoyed much success for two-photon excitation, but has not previously been applied to one-photon excitation. This corrected PCH model is able to resolve fluorescent species with different degrees of brightness, for example, a mixture of tetramethylrhodamine-5′-maleimide and Cy3-maleimide (see gr...

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Publication status:
Published

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Publisher copy:
10.1002/cphc.200300824

Authors


Perroud, TD More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Journal:
Chemphyschem : a European journal of chemical physics and physical chemistry
Volume:
4
Issue:
10
Pages:
1121-1123
Publication date:
2003-10-05
DOI:
EISSN:
1439-7641
ISSN:
1439-4235
URN:
uuid:a3be9db6-34d7-46fc-9b70-c2800f56fb6b
Source identifiers:
59579
Local pid:
pubs:59579

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