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Novel multiplex real-time PCR assays reveal a high prevalence of diarrhoeagenic Escherichia coli pathotypes in healthy and diarrhoeal children in the south of Vietnam

Abstract:

Background: Diarrhoeagenic Escherichia coli (DEC) infections are common in children in low-middle income countries (LMICs). However, detecting the various DEC pathotypes is complex as they cannot be differentiated by classical microbiology. We developed four multiplex real-time PCR assays were to detect virulence markers of six DEC pathotypes; specificity was tested using DEC controls and other enteric pathogens. PCR amplicons from the six ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher:
BioMed Central Publisher's website
Journal:
BMC Microbiology Journal website
Volume:
20
Article number:
192
Publication date:
2020-07-03
Acceptance date:
2020-06-25
DOI:
EISSN:
1471-2180
Pmid:
32620076
Pubs id:
1117105
Local pid:
pubs:1117105

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