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In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering

Abstract:

RNA regulatory elements (RREs) are an important yet relatively under-explored facet of gene regulation. Deciphering the prevalence and functional impact of this post-transcriptional control layer requires technologies for disrupting RREs without perturbing cellular homeostasis. Here we describe genome-engineering based evaluation of RNA regulatory element activity (GenERA), a CRISPR-Cas9 platform for in situ high-content functional analysis of RREs. We use GenERA to survey the entire regulato...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/s41467-017-00686-2

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Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
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Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
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Department:
Oxford, MSD, RDM, Weatherall Insti. of Molecular Medicine
Vitsios, DM More by this author
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Funding agency for:
Ferry, QRV
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Funding agency for:
Michaels, YS
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Funding agency for:
Michaels, YS
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Communications Journal website
Volume:
8
Pages:
Article: 2109
Publication date:
2017-12-13
Acceptance date:
2017-07-19
DOI:
ISSN:
2041-1723
Pubs id:
pubs:713683
URN:
uri:a38965cf-7752-4775-86eb-71424c193fb6
UUID:
uuid:a38965cf-7752-4775-86eb-71424c193fb6
Local pid:
pubs:713683

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