Cloning and heterologous expression of Plasmodium ovale dihydrofolate reductase-thymidylate synthase gene.

Journal article

Plasmodial bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) is a validated antimalarial drug target. In this study, expression of the putative dhfr-ts of Plasmodium ovale rescued the DHFR chemical knockout and a TS null bacterial strain, demonstrating its DHFR and TS catalytic functions. PoDHFR-TS was expressed in Escherichia coli BL21 (DE3) and affinity purified by Methotrexate Sepharose column. Biochemical and enzyme kinetics characterizations indicated that PoDHFR-TS is ...

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Authors: Tirakarn, S; Riangrungroj, P; Kongsaeree, P; Imwong, M; Yuthavong, Y

• Journal: Parasitology international
• Volume: 61
• Issue: 2
• Pages: 324-332
• Publication date: 2012-06-01
• DOI: 10.1016/j.parint.2011.12.004
• EISSN: 1873-0329
• ISSN: 1383-5769
• Language: English
• Keywords: Plasmodium ovale; Genetic Complementation Test; Amino Acid Sequence; Thymidylate Synthase; Cloning, Molecular; Escherichia coli; Sequence Analysis, DNA; Antimalarials; Folic Acid Antagonists; Tetrahydrofolate Dehydrogenase; Sequence Alignment; Protozoan Proteins; Proguanil; Kinetics; Molecular Sequence Data; Hydrogen-Ion Concentration; Pyrimethamine; Multienzyme Complexes; Triazines