Estimation of apparent tumor vascular permeability from multiphoton fluorescence microscopic images of P22 rat sarcomas in vivo.

Journal article

OBJECTIVE: To develop an image processing-based method to quantify the rate of extravasation of fluorescent contrast agents from tumor microvessels, and to investigate the effect of the tumor vascular disrupting agent combretastatin A-4-P (CA-4-P) on apparent tumor vascular permeability to 40 kDa fluorescein isothiocyanate (FITC) labeled dextran. METHODS: Extravasation of FITC-dextran was imaged in 3 dimensions over time within P22 sarcomas growing in dorsal skin flap "window chambers" in BDI...

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Authors: Reyes-Aldasoro, C; Wilson, I; Prise, V; Barber, P; Ameer-Beg, M

• Publication status: Published
• Journal: Microcirculation (New York, N.Y. : 1994)
• Volume: 15
• Issue: 1
• Pages: 65-79
• Publication date: 2008-01-01
• DOI: 10.1080/10739680701436350
• EISSN: 1549-8719
• ISSN: 1073-9688
• Language: English
• Keywords: Animals; Neoplasms, Connective Tissue; Neovascularization, Pathologic; Dextrans; Rats; Rats, Inbred Strains; Sarcoma; Capillary Permeability; Algorithms; Image Processing, Computer-Assisted; Fluorescein-5-isothiocyanate; Microscopy, Fluorescence; Neoplasm Transplantation; Microcirculation