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FCS in STED microscopy: studying the nanoscale of lipid membrane dynamics.

Abstract:

Details of molecular membrane dynamics in living cells such as lipid-protein interactions or the incorporation of molecules into lipid "rafts" are often hidden to the observer because of the limited spatial resolution of conventional far-field optical microscopy. Fortunately, the superior spatial resolution of far-field stimulated-emission-depletion (STED) nanoscopy allows gaining new insights. Applying fluorescence correlation spectroscopy (FCS) in focal spots continuously tuned down to 30 n...

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Publication status:
Published

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Authors


Mueller, V More by this author
Honigmann, A More by this author
Ringemann, C More by this author
Schwarzmann, G More by this author
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Journal:
Methods in enzymology
Volume:
519
Pages:
1-38
Publication date:
2013
DOI:
EISSN:
1557-7988
ISSN:
0076-6879
URN:
uuid:a24ed2a6-fa26-4a0a-8df3-2b8e693b7e3e
Source identifiers:
369906
Local pid:
pubs:369906

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