Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.

Journal article

A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.

Authors: Tanomsing, N; Imwong, M; Theppabutr, S; Pukrittayakamee, S; Day, N

• Publication status: Published
• Journal: Journal of clinical microbiology
• Volume: 48
• Issue: 10
• Pages: 3735-3737
• Publication date: 2010-10-01
• DOI: 10.1128/jcm.00898-10
• EISSN: 1098-660X
• ISSN: 0095-1137
• Language: English
• Keywords: Humans; Polymerase Chain Reaction; Malaria; Thymidylate Synthase; Sequence Analysis, DNA; Tetrahydrofolate Dehydrogenase; Molecular Sequence Data; DNA, Protozoan; Plasmodium; Parasitology; DNA, Intergenic; Sensitivity and Specificity