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Femtosecond near-infrared lasers as a novel tool for non-invasive real-time high-resolution time-lapse imaging of chloroplast division in living bundle sheath cells of Arabidopsis.

Abstract:

Non-linear excitation of fluorophores by contemporaneous absorption of two or more near-infrared (NIR) photons following diffraction-limited focusing with high-numerical-aperture objectives circumvents out-of-focus fluorescence (without a confocal pinhole) and spatially limits photobleaching and photodamage to the minute sub-femtoliter focal volume. This is in contrast to optical events in conventional confocal imaging systems using ultraviolet (UV) or visible laser sources wherein the entire...

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Publication status:
Published

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Publisher copy:
10.1007/s004250100597

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Journal:
Planta
Volume:
214
Issue:
1
Pages:
1-10
Publication date:
2001-11-05
DOI:
EISSN:
1432-2048
ISSN:
0032-0935
URN:
uuid:a2279b52-e9d8-4b9c-bf9c-fb8d135ef002
Source identifiers:
108457
Local pid:
pubs:108457

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