Microcarriers for high-pressure freezing and cryosectioning of adherent cells.

Journal article

A method is described employing microcarrier spheres of cross-linked dextran for obtaining ultra- and semithin vitreous sections from high-pressure frozen anchorage-dependent (mammalian) cells. Avoiding trypsination or scraping cells off from the culture surface, the presented approach allows for cryoimmobilization, cryosectioning and cryoelectron microscopy/tomography of frozen-hydrated cells in an unperturbed manner which is important to preserve the native state of, for instance, the cytos...

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Authors: Hagen, C; Grünewald, K

• Publication status: Published
• Journal: Journal of microscopy
• Volume: 230
• Issue: Pt 2
• Pages: 288-296
• Publication date: 2008-05-01
• DOI: 10.1111/j.1365-2818.2008.01987.x
• EISSN: 1365-2818
• ISSN: 0022-2720
• Language: English
• Keywords: Animals; Cell Culture Techniques; Cryoelectron Microscopy; Cryoultramicrotomy; Dextrans; Cell Adhesion; Cercopithecus aethiops; Freezing; Frozen Sections; Vero Cells; Herpesvirus 1, Human; Microspheres; Cryopreservation; Microscopy, Fluorescence