Journal article icon

Journal article

Structural basis for the properties of two single-site proline mutants of CYP102A1 (P450BM3).

Abstract:

The crystal structures of the haem domains of Ala330Pro and Ile401Pro, two single-site proline variants of CYP102A1 (P450(BM3)) from Bacillus megaterium, have been solved. In the A330P structure, the active site is constricted by the relocation of the Pro329 side chain into the substrate access channel, providing a basis for the distinctive C-H bond oxidation profiles given by the variant and the enhanced activity with small molecules. I401P, which is exceptionally active towards non-natural ...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1002/cbic.201000421

Authors


Whitehouse, CJ More by this author
Rowlatt, BC More by this author
Strong, AJ More by this author
Expand authors...
Journal:
Chembiochem : a European journal of chemical biology
Volume:
11
Issue:
18
Pages:
2549-2556
Publication date:
2010-12-05
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
URN:
uuid:a1e57133-b9e5-4a60-bd0a-d1f9a383fa5d
Source identifiers:
103605
Local pid:
pubs:103605

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP