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Development of a high-resolution melting method for mutation detection in familial hypercholesterolaemia patients.

Abstract:

AIMS: Current screening methods, such as single strand conformational polymorphism (SSCP) and denaturing high performance liquid chromatography (dHPLC) that are used for detecting mutations in familial hypercholesterolaemia (FH) subjects are time consuming, costly and only 80-90% sensitive. Here we have tested high-resolution melt (HRM) analysis for mutation detection using the Rotor-Gene(6000) realtime rotary analyser. Methods and subjects Polymerase chain reaction and melt conditions (HRM) ...

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Publisher copy:
10.1258/acb.2009.009076

Authors


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Journal:
Annals of clinical biochemistry
Volume:
47
Issue:
Pt 1
Pages:
44-55
Publication date:
2010-01-05
DOI:
EISSN:
1758-1001
ISSN:
0004-5632
URN:
uuid:a1909100-d674-4b4c-bc72-0bf8ceb688b9
Source identifiers:
72825
Local pid:
pubs:72825

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