Structural and functional basis for lipid synergy on the activity of the antibacterial peptide ABC transporter McjD

Journal article

The lipid bilayer is a dynamic environment that consists of a mixture of lipids with different properties that regulate the function of membrane proteins; these lipids are either annular, masking the protein hydrophobic surface, or specific lipids, essential for protein function. In this study, using tandem mass spectrometry, we have identified specific lipids associated with the Escherichia coli ABC transporter McjD, which translocates the antibacterial peptide MccJ25. Using non-denaturing m...

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Authors: Mehmood, S; Corradi, V; Choudhury, H; Hussain, R; Becker, P

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Society for Biochemistry and Molecular Biology
• Journal: Journal of Biological Chemistry
• Volume: 291
• Issue: 41
• Pages: 21656 –21668
• Publication date: 2017-08-23
• Acceptance date: 2016-08-23
• DOI: 10.1074/jbc.M116.732107
• EISSN: 1083-351X
• ISSN: 0021-9258
• Pmid: 27555327
• Language: English
• Keywords: structure-function; non-denaturing mass spectrometry; structural biology; circular dichroism (CD); molecular dynamics; delipidation; membrane transport; membrane protein; lipid; ABC transporter