Biochemical characterization and DNA repair pathway interactions of Mag1-mediated base excision repair in Schizosaccharomyces pombe.

Journal article

The Schizosaccharomyces pombe mag1 gene encodes a DNA repair enzyme with sequence similarity to the AlkA family of DNA glycosylases, which are essential for the removal of cytotoxic alkylation products, the premutagenic deamination product hypoxanthine and certain cyclic ethenoadducts such as ethenoadenine. In this paper, we have purified the Mag1 protein and characterized its substrate specificity. It appears that the substrate range of Mag1 is limited to the major alkylation products, such ...

Expand abstract

Authors: Alseth, I; Osman, F; Korvald, H; Tsaneva, I; Whitby, M

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 33
• Issue: 3
• Pages: 1123-1131
• Publication date: 2005-01-01
• DOI: 10.1093/nar/gki259
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: Multienzyme Complexes; Mutagens; Mutation; DNA Glycosylases; Substrate Specificity; DNA Adducts; Schizosaccharomyces pombe Proteins; Methyl Methanesulfonate; DNA Repair; Schizosaccharomyces; Purines; DNA-(Apurinic or Apyrimidinic Site) Lyase; Recombination, Genetic; Endodeoxyribonucleases