An aqueous H+ permeation pathway in the voltage-gated proton channel Hv1.

Journal article

Hv1 voltage-gated proton channels mediate rapid and selective transmembrane H(+) flux and are gated by both voltage and pH gradients. Selective H(+) transfer in membrane proteins is commonly achieved by Grotthuss proton 'hopping' in chains of ionizable amino acid side chains and intraprotein water molecules. To identify whether ionizable residues are required for proton permeation in Hv1, we neutralized candidate residues and measured expressed voltage-gated H(+) currents. Unexpectedly, charg...

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Authors: Ramsey, I; Mokrab, Y; Carvacho, I; Sands, Z; Sansom, M

• Publication status: Published
• Journal: Nature structural and molecular biology
• Volume: 17
• Issue: 7
• Pages: 869-875
• Publication date: 2010-07-01
• DOI: 10.1038/nsmb.1826
• EISSN: 1545-9985
• ISSN: 1545-9993
• Language: English
• Keywords: Water; Protein Conformation; Cell Line; Mutation; Ion Channels; Humans; Sequence Alignment; Amino Acid Sequence; Mice; Molecular Sequence Data; Animals; Protons; Molecular Dynamics Simulation