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Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I. Changes in [4Fe-4S] cluster reduction potential and reactivity.

Abstract:

We have used site-directed mutagenesis to obtain two variants of Azotobacter vinelandii ferredoxin I (AvFdI), whose x-ray structures are now available. In the C20A protein, a ligand to the [4Fe-4S] cluster was removed whereas in the C24A mutant a free cysteine next to that cluster was removed. Like native FdI, both mutants contain one [4Fe-4S] cluster and one [3Fe-4S] cluster. The structure of C24A is very similar to that of native FdI, while the structure of C20A is rearranged in the region ...

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Publication status:
Published

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Journal:
The Journal of biological chemistry
Volume:
266
Issue:
32
Pages:
21563-21571
Publication date:
1991-11-05
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:a0e671d9-63a6-4638-9fb7-cba03bd8aafe
Source identifiers:
46002
Local pid:
pubs:46002

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