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Journal article

Central pore residues mediate the p97/VCP activity required for ERAD.

Abstract:

The AAA-ATPase p97/VCP facilitates protein dislocation during endoplasmic reticulum-associated degradation (ERAD). To understand how p97/VCP accomplishes dislocation, a series of point mutants was made to disrupt distinguishing structural features of its central pore. Mutants were evaluated in vitro for ATPase activity in the presence and absence of synaptotagmin I (SytI) and in vivo for ability to process the ERAD substrate TCRalpha. Synaptotagmin induces a 4-fold increase in the ATPase acti...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Ludwig Institute
Role:
Author
Journal:
Molecular cell
Volume:
22
Issue:
4
Pages:
451-462
Publication date:
2006-05-05
DOI:
EISSN:
1097-4164
ISSN:
1097-2765
URN:
uuid:a0d70c1b-7855-41d4-bb38-f3dab8b71bae
Source identifiers:
53496
Local pid:
pubs:53496

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