Journal article
CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape
- Abstract:
-
CRISPRi, an adapted CRISPR-Cas9 system, is proposed to act as a strand-specific roadblock to repress transcription in eukaryotic cells using guide RNAs (sgRNAs) to target catalytically inactive Cas9 (dCas9) and offers an alternative to genetic interventions for studying pervasive antisense transcription. Here, we successfully use click chemistry to construct DNA templates for sgRNA expression and show, rather than acting simply as a roadblock, sgRNA/dCas9 binding creates an environment that i...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
Funding
+ Biotechnology and Biological Sciences Research Council
More from this funder
Funding agency for:
Brown, T
Mellor, J
Grant:
BB/J001694/2
BB/J001694/2
Bibliographic Details
- Publisher:
- eLife Sciences Publications Publisher's website
- Journal:
- eLife Journal website
- Volume:
- 6
- Article number:
- e29878
- Publication date:
- 2017-10-23
- Acceptance date:
- 2017-10-22
- DOI:
- ISSN:
-
2050-084X
- Pmid:
-
29058669
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:738935
- UUID:
-
uuid:a0b6f465-40a9-46c1-aac9-f34828e31883
- Local pid:
- pubs:738935
- Source identifiers:
-
738935
- Deposit date:
- 2017-11-14
Terms of use
- Copyright holder:
- Howe et al
- Copyright date:
- 2017
- Notes:
-
Copyright
© 2017 Howe et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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