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A triazole linkage that mimics the DNA phosphodiester group in living systems

Abstract:

We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modi...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1017/s0033583515000141

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Role:
Author
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Grant:
BB/J001694/1: ‘Extending the Boundaries of Nucleic Acid Chemistry.’
Publisher:
Cambridge University Press Publisher's website
Journal:
Quarterly Reviews of Biophysics Journal website
Volume:
48
Issue:
4
Pages:
429-436
Publication date:
2015-11-01
DOI:
EISSN:
1469-8994
ISSN:
0033-5835
Source identifiers:
578783
Language:
English
Keywords:
Pubs id:
pubs:578783
UUID:
uuid:a0b1ffdb-f5bb-4731-935f-53244bc6e887
Local pid:
pubs:578783
Deposit date:
2016-03-01

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