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Polarity-sensitive probes for superresolution stimulated emission depletion microscopy

Abstract:

The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed (or ordered). Polarity sensitive dyes are powerful tools to characterize such lipid membrane order, employing for example confocal and two-photon microscopy. The investigation of potential lipid nanodomains, however, requires the use of super resolution microscopy. Here, we test the p...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bpj.2017.06.050

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM - Investigative Medicine Division
Role:
Author
ORCID:
0000-0002-4915-388X
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Sub department:
Weatherall Institute of Molecular Medicine
Role:
Author
Publisher:
Elsevier Publisher's website
Journal:
Biophysical Journal Journal website
Volume:
113
Issue:
6
Pages:
1321-1330
Publication date:
2017-07-19
Acceptance date:
2017-06-28
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
Pubs id:
pubs:894267
UUID:
uuid:a07eaa17-e205-4844-a4ae-a7b18755c525
Local pid:
pubs:894267
Source identifiers:
894267
Deposit date:
2019-02-05

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