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A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples

Abstract:

To date, very large scale sequencing of many clinically important RNA viruses has been complicated by their high population molecular variation, which creates challenges for polymerase chain reaction and sequencing primer design. Many RNA viruses are also difficult or currently not possible to culture, severely limiting the amount and purity of available starting material. Here, we describe a simple, novel, high-throughput approach to Norovirus and Hepatitis C virus whole genome sequence dete...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1371/journal.pone.0066129

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Department:
St Johns College
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Wellcome Trust More from this funder
Department of Health More from this funder
Medical Research Council More from this funder
Publisher:
Public Library of Science Publisher's website
Journal:
PloS one Journal website
Volume:
8
Issue:
6
Pages:
e66129
Publication date:
2013-06-10
DOI:
EISSN:
1932-6203
Pubs id:
pubs:405886
URN:
uri:9fd4b0fc-c164-4311-93ef-6a6bd5ed0638
UUID:
uuid:9fd4b0fc-c164-4311-93ef-6a6bd5ed0638
Local pid:
pubs:405886

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