Isolation of adult porcine islets of Langerhans.

Journal article

While human islet allotransplantation is limited due to the shortage of donors, pig islet xenotransplantation appears to be a possible alternative. Enzymatic digestion is the method most commonly used for adult pig islet isolation, but since the description of the semiautomated isolation technique in the late 1980s, yields of pig islet isolations have not increased significantly. This review article is intended to collate published information on adult pig islet isolation to provide the reade...

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Authors: Toso, C; Brandhorst, D; Oberholzer, J; Triponez, F; Bühler, L

• Journal: Cell transplantation
• Volume: 9
• Issue: 3
• Pages: 297-305
• Publication date: 2000-01-01
• EISSN: 1555-3892
• ISSN: 0963-6897
• Language: English
• Keywords: Transplantation, Heterologous; Humans; Pancreas; Islets of Langerhans; Cell Separation; Islets of Langerhans Transplantation; Ischemia; Swine; Animals; Collagenases; Tissue and Organ Procurement; Tissue and Organ Harvesting