Characterization of Sp1, AP-1, CBF and KRC binding sites and minisatellite DNA as functional elements of the metastasis-associated mts1/S100A4 gene intronic enhancer.

Journal article

The mts1/S100A4 gene encodes a small acidic calcium-binding protein that is expressed in a cell-specific manner in development, tumorigenesis and certain tissues of adult mice. A composite enhancer that is active in murine mammary adenocarcinoma cells was previously identified in the first intron of the mts1/S100A4 gene. Here we present a detailed analysis of the structure and function of this enhancer in the Mts1/S100A4-expressing CSML100 and non-expressing CSML0 mouse adenocarcinoma cell li...

Expand abstract

Authors: Cohn, M; Hjelmsø, I; Wu, L; Guldberg, P; Lukanidin, E

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 29
• Issue: 16
• Pages: 3335-3346
• Publication date: 2001-08-01
• DOI: 10.1093/nar/29.16.3335
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: DNA Footprinting; DNA; Animals; Allosteric Site; CREB-Binding Protein; DNA-Binding Proteins; Genes, p16; Organ Specificity; Base Sequence; Introns; Nuclear Proteins; Neoplasm Metastasis; Molecular Sequence Data; Transcription Factor AP-1; Mice; Genes, Viral; Mutation; Response Elements; Promoter Regions, Genetic; Transcription Factors; Tumor Cells, Cultured; Trans-Activators; Sp1 Transcription Factor; Gene Expression Regulation, Neoplastic; Minisatellite Repeats; Enhancer Elements, Genetic