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Characterization of the RstB2 protein, the DNA-binding protein of CTXϕ phage from Vibrio cholerae.

Abstract:

The low abundant protein RstB2, encoded in the RS2 region of CTXϕ, is essential for prophage formation. However, the only biochemical activity so far described is the single/double-stranded DNA-binding capacity of that protein. In this paper, a recombinant RstB2 (rRstB2) protein was overexpressed in E. coli with a yield of 58.4 mg l(-1) in shaken cultures, LB broth. The protein, purified to homogeneity, showed an identity with rRstB2 by peptide mass fingerprinting. The apparent molecular weig...

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Publication status:
Published

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Publisher copy:
10.1007/s11262-014-1053-0

Authors


Marrero, K More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Condensed Matter Physics
Journal:
Virus genes
Volume:
48
Issue:
3
Pages:
518-527
Publication date:
2014-06-05
DOI:
EISSN:
1572-994X
ISSN:
0920-8569
URN:
uuid:9f3b7dc0-4aa4-43fc-93b3-093009f22c3f
Source identifiers:
457662
Local pid:
pubs:457662

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