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A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations

Abstract:

The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alph...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41598-023-30754-1

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Role:
Contributor
Role:
Contributor
Publisher:
Springer Nature
Journal:
Scientific Reports More from this journal
Volume:
13
Issue:
1
Article number:
3887
Place of publication:
England
Publication date:
2023-03-08
Acceptance date:
2023-02-28
DOI:
EISSN:
2045-2322
ISSN:
2045-2322
Pmid:
36890186
Language:
English
Pubs id:
1335864
Local pid:
pubs:1335864
Deposit date:
2024-04-16

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