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Characterising electrosprayed biomolecules using tandem-MS - the noncovalent GroEL chaperonin assembly

Abstract:

It is now possible to transfer large protein complexes intact into the gas phase using nano-electrospray ionisation (ESI) and to investigate their stoichiometry in a mass spectrometer. Using as a model assembly the noncovalent 14mer (ca. 800 kDa) of the chaperonin GroEL from Escherichia coli we show that the measured mass of the assembly is higher than expected from the sum of the components and explore parameters of ion activation that affect this 'noncovalent mass shift'. Under optimal deso...

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Publication status:
Published

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Publisher copy:
10.1016/j.ijms.2004.05.010

Authors


Robinson, CV More by this author
Journal:
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume:
236
Issue:
1-3
Pages:
25-32
Publication date:
2004-08-05
DOI:
ISSN:
1387-3806
URN:
uuid:9f31f9da-c4fa-4b65-969e-71dc1de74465
Source identifiers:
59365
Local pid:
pubs:59365

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