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Addition of functional human telomeres to YACs.

Abstract:

Linear mammalian artificial chromosomes (MACs) will require functional telomeres, a centromere and the ability to replicate autonomously. We are investigating the possibility of developing MACs from yeast artificial chromosomes (YACs). Retrofitting vectors have been constructed to replace YAC telomeres with cloned human telomeric DNA. A modified YAC was introduced into mammalian cells by spheroplast fusion and the frequency with which the retrofitted human telomeric DNA seeded the formation o...

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Publication status:
Published

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Publisher copy:
10.1093/hmg/3.8.1383

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Journal:
Human molecular genetics
Volume:
3
Issue:
8
Pages:
1383-1386
Publication date:
1994-08-05
DOI:
EISSN:
1460-2083
ISSN:
0964-6906
URN:
uuid:9f1516f5-8f6d-4e9a-ac69-eaee6de251ef
Source identifiers:
53556
Local pid:
pubs:53556

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