Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae.

Journal article

The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex. Deletion of SGS1, TOP3, or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution. To investigate the causes of this synthetic lethality, we isolated a temperature-sensitive mutant of the RMI1 strain, referred to as the rmi1-1 mutant. At the restrictive temperature, this mutant p...

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Authors: Ashton, T; Mankouri, H; Heidenblut, A; McHugh, P; Hickson, I

• Journal: Molecular and cellular biology
• Volume: 31
• Issue: 9
• Pages: 1921-1933
• Publication date: 2011-05-01
• DOI: 10.1128/mcb.01130-10
• EISSN: 1098-5549
• ISSN: 0270-7306
• Language: English
• Keywords: Saccharomyces cerevisiae; DNA, Cruciform; Holliday Junction Resolvases; DNA, Bacterial; Saccharomyces cerevisiae Proteins; Escherichia coli Proteins; Temperature; Recombination, Genetic; Mutation; DNA-Binding Proteins; Flap Endonucleases; Endonucleases; Methyl Methanesulfonate