Use of electrospray mass spectrometry to directly observe an acyl enzyme intermediate in beta-lactamase catalysis.
- Electrospray mass spectrometry was used to directly observe intact acyl enzyme complexes formed between a class C beta-lactamase (from Enterobacter cloacae P99) and four poor substrates/inhibitors. In each case the molecular weight difference between the unreacted and the reacted beta-lactamase was consistent with the formation of an acyl enzyme.
- Publication status:
- Publisher copy:
- Copyright date: