The effects of a low extracellular concentration of potassium on the activity and numbers of Na+/K+ pumps in an EB-virus transformed human lymphocyte cell line.

Journal article

The BM1A EB-virus transformed human lymphocyte cell line contains approximately 950,000 Na+/K(+)-ATPase sites per cell. The turnover number of each site is approx. 2240 molecules of rubidium per min. When cells are exposed to a low extracellular concentration of potassium the intracellular concentration of sodium rises, and the cells respond in the short term by increasing the Vmax of 86Rb+ uptake. In the longer term the cells respond by increasing both the Vmax of 86Rb+ uptake and the Bmax o...

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Authors: Kennedy, D; Aronson, J; Bloomfield, J; Grahame-Smith, D

• Publication status: Published
• Journal: Biochimica et biophysica acta
• Volume: 1027
• Issue: 3
• Pages: 218-224
• Publication date: 1990-09-01
• DOI: 10.1016/0005-2736(90)90310-k
• ISSN: 0006-3002
• Language: English
• Keywords: Monensin; Humans; Potassium; Lymphocytes; Sodium Channels; Herpesvirus 4, Human; gamma-Glutamyltransferase; Sodium-Potassium-Exchanging ATPase; Rubidium; Ouabain; Cell Transformation, Viral; Thymidine; Biological Transport, Active; Potassium Channels; 5'-Nucleotidase