Monoclonal antibody to human platelet glycoprotein I. I. Immunological studies.

Journal article

A monoclonal hybridoma antibody specific for platelet glycoprotein I complex is described. The nature od the antigen was determined by demonstration that it was chymotrypsin sensitive and gave a peak at 150 000 daltons on SDS-PAGE after immunoprecipitation. The expression of the antigen is restricted to platelets and megakaryocytes with at least 1.6 x 10(4) molecules of antigen per platelet. The antibody failed to bind to platelets from patients with Bernard Soulier syndrome, where there is known to be a deficiency of glycoprotein Ib/Is expression. Binding to platelets from patients with Glanzmann's thrombasthenia was normal.

Authors: Mcmichael, A; Rust, N; Pilch, JR; Sochynsky, R; Morton, J

• Publication status: Published
• Journal: British journal of haematology
• Volume: 49
• Issue: 4
• Pages: 501-509
• Publication date: 1981-12-01
• DOI: 10.1111/j.1365-2141.1981.tb07258.x
• EISSN: 1365-2141
• ISSN: 0007-1048
• Language: English
• Keywords: Antibody Specificity; Glycoproteins; Humans; Antigens; Precipitin Tests; Platelet Membrane Glycoproteins; Membrane Proteins; Blood Platelets; Antigen-Antibody Reactions; Tissue Distribution; Animals; Blood Platelet Disorders; Antibodies, Monoclonal; Mice