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Quantifying the syncytialisation of human placental trophoblast BeWo cells grown in vitro.

Abstract:

We have generated lines of BeWo cells that constitutively and stably express either histone H2B tagged with the green fluorescent protein (GFP), or the mitochondrial targeting sequence of subunit VIII of cytochrome c oxidase fused with a red fluorescent protein; one line has nuclei that fluoresce green, the other mitochondria that fluoresce red. Expression of these tagged proteins has no effect on the rates of DNA, RNA and protein synthesis, or on the amounts of human chorionic gonadotropin (...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Institution:
University of Oxford
Department:
Oxford, MSD, Obstetrics and Gynaecology
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Journal:
Biochimica et biophysica acta
Volume:
1640
Issue:
1
Pages:
25-31
Publication date:
2003-04-05
DOI:
ISSN:
0006-3002
URN:
uuid:9e855903-fe9b-42a0-ad74-be744d621859
Source identifiers:
24165
Local pid:
pubs:24165

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