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Crystal structure of the vanadate-inhibited Ca(2+)-ATPase

Abstract:

Vanadate is the hallmark inhibitor of the P-type ATPase family; however, structural details of its inhibitory mechanism have remained unresolved. We have determined the crystal structure of sarcoplasmic reticulum Ca(2+)-ATPase with bound vanadate in the absence of Ca(2+). Vanadate is bound at the catalytic site as a planar VO3(-) in complex with water and Mg(2+) in a dephosphorylation transition-state-like conformation. Validating bound VO3(-) by anomalous difference Fourier maps using long-w...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.str.2016.02.018

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Publisher:
Elsevier Publisher's website
Journal:
Structure Journal website
Volume:
24
Issue:
4
Pages:
617-623
Publication date:
2016-04-05
Acceptance date:
2016-02-25
DOI:
ISSN:
0969-2126 and 1878-4186
Pmid:
27050689
Language:
English
Keywords:
Pubs id:
pubs:618919
UUID:
uuid:9e389297-7d92-47f5-9731-f9a506c1b79b
Local pid:
pubs:618919
Deposit date:
2016-11-03

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