Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination.

Journal article

Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. For two well-defined Pol II-transcribed genes, CYC1 and PMA1, we demonstrate that both Rat1p exonuclease and Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with Pol II, following mRNA 3' end processing. Similarly, Pol I termination relies on prior Rnt1p cleavage at...

Expand abstract

Authors: Kawauchi, J; Mischo, H; Braglia, P; Rondon, A; Proudfoot, N

• Publication status: Published
• Journal: Genes and development
• Volume: 22
• Issue: 8
• Pages: 1082-1092
• Publication date: 2008-04-01
• DOI: 10.1101/gad.463408
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: RNA Helicases; DNA, Ribosomal; DNA Helicases; RNA Polymerase II; Proton-Translocating ATPases; Fungal Proteins; Exoribonucleases; Terminator Regions, Genetic; RNA Polymerase I; Transcription, Genetic; Cytochromes c; Saccharomyces cerevisiae; Models, Biological; Saccharomyces cerevisiae Proteins