Protective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8(+)- and CD4(+)-T-cell epitopes from antigen 85A.

Journal article

Immunization with DNA followed by modified vaccinia virus Ankara strain, both expressing the antigen 85A, induced both CD4(+)- and CD8(+)-T-cell responses in BALB/c mice. Following challenge with Mycobacterium tuberculosis, this prime-boost regimen produced protection equivalent to that conferred by Mycobacterium bovis BCG. Following immunization with dendritic cells pulsed with an antigen 85A CD4(+)- or CD8(+)-restricted epitope, alone or in combination, copresentation of both epitopes on the same dendritic cell was required for protection, demonstrating that induced CD8(+) T cells can play a protective role against tuberculosis.

Authors: Mcshane, H; Behboudi, S; Goonetilleke, N; Brookes, R; Hill, A

• Publication status: Published
• Journal: Infection and immunity
• Volume: 70
• Issue: 3
• Pages: 1623-1626
• Publication date: 2002-03-01
• DOI: 10.1128/iai.70.3.1623-1626.2002
• EISSN: 1098-5522
• ISSN: 0019-9567
• Language: English
• Keywords: Epitopes; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; BCG Vaccine; Mice, Inbred BALB C; Acyltransferases; Tuberculosis; Female; Antigen Presentation; Mice; Vaccination; Molecular Sequence Data; Animals; Dendritic Cells; Antigens, Bacterial; Amino Acid Sequence