Journal article icon

Journal article

Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation.

Abstract:

Fluorescence resonance energy transfer (FRET) between a donor (D) and an acceptor (A) at the single-molecule level currently provides qualitative information about distance, and quantitative information about kinetics of distance changes. Here, we used the sorting ability of confocal microscopy equipped with alternating-laser excitation (ALEX) to measure accurate FRET efficiencies and distances from single molecules, using corrections that account for cross-talk terms that contaminate the FRE...

Expand abstract

Actions


Access Document


Publisher copy:
10.1529/biophysj.104.054114

Authors


Kapanidis, AN More by this author
Michalet, X More by this author
Mukhopadhyay, J More by this author
Expand authors...
Journal:
Biophysical journal
Volume:
88
Issue:
4
Pages:
2939-2953
Publication date:
2005-04-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:9c8e8d35-9391-4bb1-8761-f56ed064cb65
Source identifiers:
29784
Local pid:
pubs:29784

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP