EM visualization of transcription by RNA polymerase II: downstream termination requires a poly(A) signal but not transcript cleavage.

Journal article

We have used EM visualization of active genes on plasmid vectors in Xenopus oocyte nuclei to investigate the relationship between poly(A) signals and RNA polymerase II transcription termination. Although a functional poly(A) signal is required for efficient termination, cotranscriptional RNA cleavage at the poly(A) site is not. Furthermore, the phenomena of termination and cotranscriptional RNA cleavage can be uncoupled, and the efficiency of both varies independently on different copies of t...

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Authors: Osheim, Y; Proudfoot, N; Beyer, A

• Publication status: Published
• Journal: Molecular cell
• Volume: 3
• Issue: 3
• Pages: 379-387
• Publication date: 1999-03-01
• DOI: 10.1016/s1097-2765(00)80465-7
• EISSN: 1097-4164
• ISSN: 1097-2765
• Language: English
• Keywords: Plasmids; Transcription Factors; Xenopus laevis; RNA, Messenger; Transcription, Genetic; Poly A; Microscopy, Electron; Promoter Regions, Genetic; Animals; Genes; RNA Polymerase II; Transcription Factor TFIIIA; Response Elements; Templates, Genetic; DNA-Binding Proteins; Oocytes