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Human DNA polymerase lambda diverged in evolution from DNA polymerase beta toward specific Mn(++) dependence: a kinetic and thermodynamic study.

Abstract:

The recently discovered human DNA polymerase lambda (DNA pol lambda) has been implicated in translesion DNA synthesis across abasic sites. One remarkable feature of this enzyme is its preference for Mn(2+) over Mg(2+) as the activating metal ion, but the molecular basis for this preference is not known. Here, we present a kinetic and thermodynamic analysis of the DNA polymerase reaction catalyzed by full length human DNA pol lambda, showing that Mn(2+) favors specifically the catalytic step o...

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Publication status:
Published

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Publisher copy:
10.1021/bi034198m

Authors


Shevelev, I More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Oncology
Villani, G More by this author
Spadari, S More by this author
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Journal:
Biochemistry
Volume:
42
Issue:
24
Pages:
7467-7476
Publication date:
2003-06-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:9adfce53-ec93-4112-a42b-fb65f27c751c
Source identifiers:
417383
Local pid:
pubs:417383

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