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Journal article

Dead cell counts during serum cultivation are underestimated by the fluorescent live/dead assay.

Abstract:

The live/dead fluorescent assay provides a quick method for assessing the proportion of live and dead cells in cell culture systems or tissues and is widely used. Dead cells are detected by the fluorescence produced when propidium iodide (PI) binds to DNA; PI and similar molecules are excluded from live cells but can penetrate dead cells because of their loss of membrane integrity. Here we investigated the effect of serum in the culture medium on the reliability of the method. We assessed via...

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Publication status:
Published

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Publisher copy:
10.1002/biot.201000254

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
Journal:
Biotechnology journal
Volume:
6
Issue:
5
Pages:
513-518
Publication date:
2011-05-05
DOI:
EISSN:
1860-7314
ISSN:
1860-6768
URN:
uuid:9a60f960-7acb-4579-b0aa-5a0dbea4528e
Source identifiers:
146683
Local pid:
pubs:146683

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