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Biochemical characterization of New Delhi metallo-β-lactamase variants reveals differences in protein stability.

Abstract:

OBJECTIVES: Metallo-β-lactamase (MBL)-based resistance is a threat to the use of most β-lactam antibiotics. Multiple variants of the New Delhi MBL (NDM) have recently been reported. Previous reports indicate that the substitutions affect NDM activity despite being located outside the active site. This study compares the biochemical properties of seven clinically reported NDM variants. METHODS: NDM variants were generated by site-directed mutagenesis; recombinant proteins were purified to near...

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Publisher copy:
10.1093/jac/dku403

Authors


Pfeffer, I More by this author
Geffen, RE More by this author
Wilkins, SE More by this author
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Journal:
The Journal of antimicrobial chemotherapy
Volume:
70
Issue:
2
Pages:
463-469
Publication date:
2014-10-05
DOI:
EISSN:
1460-2091
ISSN:
0305-7453
URN:
uuid:9a4408ca-9e36-44d0-849f-11372bcee45c
Source identifiers:
487219
Local pid:
pubs:487219

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