Effects of osmotic and cold shock on adherent human mesenchymal stem cells during cryopreservation.

Journal article

Cryopreservation is one of the most practical methods for the long-term storage of cell-matrix systems to ensure off-shelf availability in tissue engineering, stem cell therapy and drug testing. The aim of this study is to investigate the effects of osmotic and cold shock caused by the procedures of cryoprotectant agent addition/removal and freezing during cryopreservation on cell viability, intracellular properties, such as filamentous actin distribution, mitochondria localization and intrac...

Expand abstract

Authors: Xu, X; Liu, Y; Cui, Z; Wei, Y; Zhang, L

• Publication status: Published
• Journal: Journal of biotechnology
• Volume: 162
• Issue: 2-3
• Pages: 224-231
• Publication date: 2012-12-01
• DOI: 10.1016/j.jbiotec.2012.09.004
• EISSN: 1873-4863
• ISSN: 0168-1656
• Language: English
• Keywords: Mesenchymal Stromal Cells; Mitochondria; Humans; Cold-Shock Response; Intracellular Space; Cell Shape; Cell Physiological Processes; Cells, Cultured; Cryopreservation; Osmotic Pressure; Hydrogen-Ion Concentration; Actins