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The DNA-binding activity of Gal4 is inhibited by methylation of the Gal4 binding site in plant chromatin

Abstract:

Derivatives of the Saccharomyces cerevisiae transcription factor Gal4 which act as effective transcription activators in yeast, Drosophila, mammalian cells and plant protoplasts are shown to direct expression from a GUS reporter construct when expressed in transgenic tobacco. However, in comparison to 35S-GUS controls, Gal4-mediated expression of the reporter gene was relatively weak and extremely variable. GUS expression was lost as plants matured and it was almost undetectable in most of th...

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Publication status:
Published

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Authors


Galweiler, L More by this author
Conlan, RS More by this author
Journal:
PLANT JOURNAL
Volume:
23
Issue:
1
Pages:
143-157
Publication date:
2000-07-05
DOI:
EISSN:
1365-313X
ISSN:
0960-7412
URN:
uuid:9916489c-ccd4-4015-9fe1-42e57aa90cbc
Source identifiers:
48893
Local pid:
pubs:48893
Language:
English
Keywords:

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